A field experiment was conducted to study the effect of Zn application methods on growth and yield parameters, Zn concentrations and Zn bioavailability in rice grains of two genotypes (NIA-Mehran and Shandar). The study revealed that zinc application had a positive impact on zinc bioavailability for humans by reducing phytic acid concentrations and affecting zinc bioavailability biomarkers in both polished and brown rice. Specifically, zinc application increased the number of productive tillers by 14%, grains per panicle by 88%, thousand grains weight by 10%, and grain yield by 30%.