In the paper, is presented the opinion of the main stakeholders to the Innovation potential of the Institute of Agricultural Economics and Information (IAEI) as a significant actor in the AKIS. In the paper, is also defined the innovation potential of the IAEI as the ability to create, execute or provide the innovation and crucial information to the Czech AKIS. In order to mapping the current position and the innovation potential of the IAEI, were used desk research and semi-structured interviews