China has put in place a series of policies to support private companies to engage in biotechnology research. This study uses data from a survey of 103 major agribusiness firms in the agricultural chemical and seed industries in China to evaluate the impact of government policies on private R&D investment in biotechnology. The results show that firms with positive profit expectation, public R&D subsidies, R&D collaboration with universities/research institutes or state-owned enterprises are more likely to embark on biotechnology research activities.