This policy brief presents a methodology for assessing agricultural innovation systems (AIS), developed and pilot tested by the Food and Agriculture Organization of the United Nations (FAO) in the context of the Tropical Agriculture Platform, a G20 initiative to develop capacities for agricultural innovation in the tropics supported by the European Union. Using participatory, multi-stakeholder methods and tools, the assessment of a country’s AIS take stock of enabling and hindering factors in innovation processes, identifies gaps and challenges, and advices on ways to strengthen the AIS.